COMPARISON OF ABTS (2,2-AZINOBIS(3-ETHYLBENZOTHIAZOLINE)-6-SULFONIC ACID) AND DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) ASSAYS TO MEASURE THE ANTIOXIDANT ACTIVITY OF ETANOL EXTRACT OF RED ALGA (Gelidium sp)

Authors

  • Isye Martiani Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Garut, Jl. Jati No. 42B, Tarogong Kaler, West Java, 44151, Indonesia
  • Ardi Rustamsyah Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Garut, Jl. Jati No. 42B, Tarogong Kaler, West Java, 44151, Indonesia
  • Tien Ellita Endah Puspitasari Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Garut, Jl. Jati No. 42B, Tarogong Kaler, West Java, 44151, Indonesia
  • Dani Sujana Diploma Program of Pharmacy, Karsa Husada Garut College of Health Sciences (STIKes Karsa Husada Garut), Jl. Nusa Indah No.24, Tarogong Kidul, West Java, 44151, Indonesia

DOI:

https://doi.org/10.52434/jifb.v15i1.3461

Keywords:

ABTS, antioxidant, DPPH, red algae

Abstract

Seaweeds have an ecological function as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar, and carrageenan) used in various food and pharmaceutical industries. This study aims to determine the antioxidant activity of red algae (Gelidium sp). Extraction was done using the maceration method using 96% ethanol and concentrated by rotary evaporation. The antioxidant activity of the extract was tested using ABTS and DPPH methods. Antioxidant activity was seen from the IC50. The results showed that IC50 from ethanol extract of red algae (Gelidium sp) with the DPPH method is 3,9154 ppm. Then, using the ABTS method, IC50 from ethanol extract of red algae (Gelidium sp) is 9,1178 ppm. This result shows that the ethanol extract from red algae (Gelidium sp) has very strong antioxidant activity (<50 ppm).

References

Rustamsyah A, Sujana D, Kusmiyati M. Review: Aktivitas Farmakologi Rumput Laut Genus Gracilaria (Rhodopyceae). Jurnal Farmasi Sains dan Terapan. 2023;10(1):38-43. doi:10.33508/jfst.v10i1.4318

Husna S, Nurlaida, Yusriadi, Utami HH, Najmah. Komponen Fitokimia dan Aktivitas Antioksidan Rumput Laut Eucheuma Cottonii di Perairan Desa Pajukukang Bantaeng. Jurnal Crystal: Publikasi Penelitian Kimia dan Terapannya. 2023;5(2):9-15.

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Published

2024-01-31

Issue

Vol. 15 No. 1 (2024): Jurnal Ilmiah Farmako Bahari

Section

Research Article

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