PATOGENITAS BAKTERI HIDROLITIK YANG DI ISOLASI DARI LIMBAH CAIR BIOMEDIS DI RSUD DR. SLAMET

Authors

  • Mamay Mamay STIKes Karsa Husada Garut
  • N. Ai Erlinawati STIKes Karsa Husada Garut
  • Zahara Farhan STIKes Karsa Husada Garut
  • Rina Herlina RSUD dr. Slamet Garut
  • Ranti Nurwantika RSUD dr. Slamet Garut
  • Elsa Zalfa Agustin STIKes Karsa Husada Garut

DOI:

https://doi.org/10.52434/jifb.v14i2.2433

Keywords:

bakteri hidrolitik, limbah cair biomedis, lipase, patogenitas, protease

Abstract

Bakteri patogen rendah sampai non patogen merupakan kriteria bakteri sebagai agen bioremediasi limbah cair biomedis. Bakteri indigen menghasilkan enzim pendegradasi komponen yang terkandung limbah biomedis cair seperti karbohidrat, protein dan lemak. Bakteri yang memetabolisme zat-zat organik dapat mengurangi berkembang biaknya mikroorganisme patogen. Selain itu, bioremediasi dengan bakteri indigen dengan bakteri patogenitas rendah dapat mengurangi bahaya kontaminasi dan infeksi dari mikroorganisme patogen. Tujuan penelitian ini mengetahui patogenitas bakteri hidrolitik yang diisolasi dari limbah cair biomedis di RSUD dr. Slamet Garut. Penelitian bersifat deskriptif menggunakan sampel limbah cair biomedis dari RSUD dr. Slamet Garut. Pengambilan sampel dilakukan dengan teknik Cluster Random Sampling pada penampungan, inlet dan outlet.  Isolasi bakteri protease dan lipase dengan media skrining susu skim agar dan rhodamin agar. Pengujian  patogenitas bakteri dengan menumbuhkan bakteri pada agar mac conkey, agar darah dan agar coklat. Pada media skrining  terisolasi bakteri penghasil protease sebanyak 89 koloni dan lipase sebanyak 76 koloni. Uji patogenitas menunjukan bakteri penghasil protease dengan patogenitas rendah 21,3%, sedang 27%, tinggi 36%, sangat tinggi 4,5%, ekstra tinggi 11,2%  dan bakteri penghasil lipase dengan patogenitas rendah 20%, sedang 45%, tinggi 30%, sangat tinggi 4% dan ekstra tinggi 1%. Dari penelitian diperoleh bakteri penghasil protease dan lipase dengan sebagian kecil memiliki patogenitas rendah sebagai kandidat agen bioremediasi.

References

Ola-Adisa EO, Mangden YPE, Sati YC, Adisa JO. Knowledge, attitudes/beliefs and practices in medical waste management-an appraisal of Jos North LGA, Plateau State, Nigeria. Int J Res Humanit Soc Stud [Internet]. 2015;2(12):43–56. Available from: https://www.ijrhss.org/pdf/v2-i12/5.pdf

Adhani R. Pengelolaan limbah medis pelayanan kesehatan [Internet]. 1st ed. Lambung Mangkurat University Press. Banjarmasin: Lambung Mangkurat University Press; 2018. 122 p. Available from: http://eprints.ulm.ac.id/2939/1/Buku Pengelolaan limbah medis pelayanan kesehatan_final_26feb2018.pdf.

Mora YS. Evaluasi dimensi unit istalasi pengolahan air limbah domestik Rumah Sakit dr. Ernaldi Bahar Kota Palembang. J Dampak [Internet]. 2015;12(2):127–36. Available from: http://jurnaldampak.ft.unand.ac.id/index.php/Dampak/article/view/53

Naini I, Dahlan H, Mohadi R. Kajian pengolahan limbah cair Rumah Sakit dr. Rivai Abdullah dan analisis resiko terhadap kesehatan lingkungan. Biosci Fak MIPA Univ Sriwij [Internet]. 2015;12(1):60–77. Available from: https://repository.unsri.ac.id/13537/1/42_Kajian_Pengolahan_Limbah_Cair_Rumah_Sakit_Kusta_Dr._Rivai_Abdullah_dan_Analisis_Risiko_terhadap_Kesehatan_Lingkungan.pdf.

Aliyanta B, Sumarlin LO, Mujab AS. Penggunaan biokompos dalam bioremediasi lahan tercemar limbah minyak bumi. J Kim Val [Internet]. 2012;2(3):430–42. Available from: https://journal.uinjkt.ac.id/index.php/valensi/article/view/114

Priadie B. Teknik bioremediasi sebagai alternatif dalam upaya pengendalian pencemaran air. J Ilmu Lingkung [Internet]. 2012;10(1):38–48. Available from: https://ejournal.undip.ac.id/index.php/ilmulingkungan/article/view/4094

Supriyatna A, Ukit U. Screening and isolation of cellulolytic bacteria from gut of black soldier flays larvae (Hermetia illucens) feeding with rice straw. Biosaintifika J Biol Biol Educ [Internet]. 2016;8(3):314–20. Available from: https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6762

Ethica SN, Muchlissin SI, Saptaningtyas R, Sabdono A. Sampling mikrobiologi limbah biomedis Rumah Sakit di Kota Semarang Jawa Tengah. Pros Nas dan Int [Internet]. 2017;1(1). Available from: https://jurnal.unimus.ac.id/index.php/psn12012010/article/view/2889

Ethica SN, Saptaningtyas R, Muchlissin SI, Sabdono A. The development method of bioremediation of hospital biomedical waste using hydrolytic bacteria. Health Technol (Berl) [Internet]. 2018;8(4). Available from: https://link.springer.com/article/10.1007/s12553-018-0232-8

A E, G G, R P, Et.al. Screening of microbes producing extracellular hydrolytic enzyme from corporation waste dumping site and house hold waste for the enhancement of bioremediation methods. IOSR J Pharm Biol Sci [Internet]. 2012;4(1):54–60. Available from: https://www.iosrjournals.org/iosr-jpbs/papers/Vol4-issue1/K0415456.pdf

Darmawati S, Muchlissin SI, Ernanto AR, Sulistyaningtyas AR, Fuad H, Rahman KMZ, et al. Pathogenicity scoring system for selection of bacterial consortium formulated as bioremediation agent of Hospital Wastewater in Central Java. In: IOP Conference Series: Earth and Environmental Science [Internet]. China: IOP Conference Series: Earth and Environmental Science; 2021. p. 28–30. Available from: https://iopscience.iop.org/article/10.1088/1755-1315/707/1/012003

Sulistiyawati I. Kuantitas total bakteri Coliform pada instalasi pengolahan limbah cair medis laboratorium klinik. J Ilm Univ Batanghari Jambi [Internet]. 2019;19(3):675–7. Available from: http://ji.unbari.ac.id/index.php/ilmiah/article/view/718/608

Pricilia S, Astuti W, Marliana E. Skrining bakteri endofit penghasil amilase, lipase dan protease dari daun Macaranga hullettii King ex Hook.f. J At [Internet]. 2018;3(2). Available from: http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/634

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Published

2023-07-31

Issue

Vol. 14 No. 2 (2023): Jurnal Ilmiah Farmako Bahari

Section

Research Article

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