Isolasi dan Karakterisasi Selulosa Bakteri dari Limbah Kulit Jeruk Peras (Citrus sinensis L. Osbeck)

Authors

  • Riza Apriani Universitas Garut
  • Nenda Hasanah Universitas Garut

DOI:

https://doi.org/10.52434/npcb.v1i1.42503

Keywords:

fermentasi, kulit jeruk peras, selulosa bakteri

Abstract

Selulosa bakteri merupakan salah satu biopolimer yang diproduksi oleh berbagai jenis bakteri, termasuk Gluconacetobacter xylinus. Dibandingkan dengan selulosa tanaman, selulosa bakteri memiliki keunggulan seperti biokompatibilitas tinggi dan daya serap air yang besar. Namun, produksi selulosa bakteri masih terkendala biaya tinggi akibat penggunaan media Hestrin–Schramm (HS) yang mahal. Untuk mengatasi hal tersebut, penelitian ini mengevaluasi potensi limbah kulit jeruk peras sebagai media alternatif pertumbuhan G. xylinus. Penelitian dilakukan dengan melakukan beberapa variasi parameter fermentasi, meliputi pH, jumlah inokulum, konsentrasi substrat, dan waktu fermentasi. Selulosa bakteri yang dihasilkan dianalisis menggunakan spektrofotometer Fourier Transform Infrared (FTIR) untuk mengidentifikasi gugus fungsional. Hasil menunjukkan bahwa kondisi optimum diperoleh pada pH 5, jumlah inokulum 15%, konsentrasi substrat 15%, dan waktu fermentasi 10 hari. Analisis FTIR mengidentifikasi adanya gugus O–H pada bilangan gelombang 3271,04 cm⁻¹ dan gugus C–O–C glikosida pada 1107,01 cm⁻¹, yang merupakan ciri khas selulosa. Hasil ini membuktikan bahwa limbah kulit jeruk berpotensi sebagai media alternatif yang efektif untuk produksi selulosa bakteri.

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Published

2025-05-31

Issue

Vol. 1 No. 1 (2025): Natural Product Chemistry Bulletin

Section

Artikel

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