EXPLORATION OF CHITINOLYTIC MICROORGANISMS FROM SEAWATER AS ANTI-CANDIDIASIS AGENTS USING FUNCTIONALIZED CHITIN SUBSTRATE DERIVED FROM GREEN MUSSEL WASTE

Authors

  • Rahadhyan Adnyanaschah Universitas Padjadjaran
  • Dzava Prawinsyah Fairus Ismail Universitas Padjadjaran
  • Bintang Satrio Mahardika Universitas Padjadjaran
  • Yolla Adellia Putri Universitas Padjadjaran
  • Nafis Shafa Universitas Padjadjaran
  • Tina Rostinawati Universitas Padjadjaran

DOI:

https://doi.org/10.52434/jifb.v16i1.41855

Keywords:

candidiasis, chitin, chitinolytic, green mussel, microorganism

Abstract

Candidiasis is a Candida sp. infection (Candida albicans in common) that happens in nails, hairs, skin, and oesophagus, with around 20-25% prevalence in Indonesia. Chitin is one of the main components of its cell wall and serves as a target for candidiasis therapy using natural degradant compounds, such as chitinase enzyme. Chitinase enzyme is collected from chitinolytic bacteria found massively in chitin-rich environments like seawater. The screening is needed to identify the varieties of bacteria with chitinolytic activity. The screening was done using green mussel waste (Perna viridis), which contains 14-35% chitin and reached 10.776,75 tons of trash in 2020. This research aims to get a chitinolytic bacteria using green mussels as a chitin substrate source and a chitinolytic bacteria with anti-candidiasis activity. This research was conducted by isolating chitin from green mussel shells, chitinolytic bacteria screening, and anti-candidiasis activity test. Results collected from 5.00, 5.08, and 5.18 km isolates were the Vibrio alginolyticus, Vibrio neocaledonicus, and Marinobacter persicus species, which had a chitinolytic index of 1.246, 1.560, 1.492 also 64.29; 61.30; 69.01 U/ml chitinase enzymatic activity. The highest anti-candidiasis activity was found in a sample 5,18 km from the coast of Kejawanan Beach based on the number of chitinase enzyme activity and the chitinolytic index.

 

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Published

2025-01-31

Issue

Vol. 16 No. 1 (2025): Jurnal Ilmiah Farmako Bahari

Section

Research Article

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